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mouse monoclonal anti collagen type ii antibody  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology mouse monoclonal anti collagen type ii antibody
    Mouse Monoclonal Anti Collagen Type Ii Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 328 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+col2a1/COL2A1+Antibody/10__56499_slash_jppres_14__1__2468-98-20-29
    Average 95 stars, based on 328 article reviews
    mouse monoclonal anti collagen type ii antibody - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
    Article Snippet: .. The sections were separately incubated with mouse anti-COL2A1 (Santa Cruz Biotechnology, Texas, USA, 1 : 50), rabbit anti-MMP-13 (Bioss, Beijing, China, 1 : 200), rabbit anti-F4/80 (Cell Signaling, 1: 400), rabbit anti-iNOS (Abcam, 1 : 100), and rabbit anti-CD206 (Abcam, 1 : 1000) overnight at 4°C. .. Then, the slides were incubated with a biotin-labeled IgG secondary antibody (Zhongshan Golden Bridge, Beijing, China) and stained with a diaminobenzidine (DAB) substrate and counterstained with hematoxylin.

    Article Title: Surface-Engineered Umbilical Cord Mesenchymal Stem Cell-Derived sEVs for Targeted Therapy of Osteoarthritis.
    Article Snippet: Small extracellular vesicles (sEVs) from human umbilical-cord-derived mesenchymal stem cells (UCMSCs) hold promise for cartilage regeneration in osteoarthritis (OA).. However, their therapeutic effectiveness is significantly limited by rapid clearance through blood and lymphatic vessels in the synovial tissue after intra-articular injection.. This study aimed to enhance the chondrocyte-targeting ability and improve the cartilage repair potential of UCMSC-derived sEVs by conjugating them with a chondrocyte-affinity peptide (CAP) using copper-free click chemistry.

    Article Title: A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development
    Article Snippet: .. Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400, Santa Cruz, sc-52659); rabbit anti-ISL1 (1:200, Abcam, ab20670); rabbit anti-LHX3 (1:250, Abcam, ab14555); rabbit anti-NKX2-1 (1:250, Abcam, ab76013); rabbit anti-NKX2-2 (1:250, Abcam, ab191077); rabbit anti-NKX6-1 (1:250, Abcam, ab221549); rabbit anti-OLIG2 (1:200, Abcam, ab109186); rabbit anti-oxytocin (1:500, Abcam, ab212193); rabbit anti-PAX6 (1:350, Abcam, ab195045); sheep anti-PITX2 (1:200, R&D, AF7388); rabbit anti-SF1 (1:200, Abcam, ab65815); rabbit anti-TBX3 (1:200, Abcam, ab99302); rabbit anti-TH1 (1:200, Abcam, ab137869); rabbit anti-TCF4 (1:100, Cell Signalling; C48H11); rabbit anti-vasopressin (1:500, Abcam, ab213707). .. Slides were then washed 3 times for 10 min each in PBT and incubated for 1 h at room temperature with fluorescent secondary antibodies in PBT in the dark, secondaries as follows: goat anti-rabbit 488 (1:500, Thermo Fisher Scientific, A11034); goat anti-mouse 488 (1:500, Thermo Fisher Scientific, A11029); donkey anti-sheep 594 (1:500, Thermo Fisher Scientific, A11016).

    Article Title: A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development
    Article Snippet: .. For PITX2 staining, donkey serum was used, Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400, Santa Cruz, sc-52659); rabbit anti-ISL1 (1:200, Abcam, ab20670); rabbit anti-LHX3 (1:250, Abcam, ab14555); rabbit anti-NKX2-1 (1:250, Abcam, ab76013); rabbit anti-NKX2-2 (1:250, Abcam, ab191077); rabbit anti-NKX6-1 (1:250, Abcam, ab221549); rabbit anti-OLIG2 (1:200, Abcam, ab109186); rabbit anti-oxytocin (1:500, Abcam, ab212193); rabbit anti-PAX6 (1:350, Abcam, ab195045); sheep anti-PITX2 (1:200, R&D, AF7388); rabbit anti-SF1 (1:200, Abcam, ab65815); rabbit anti-TBX3 (1:200, Abcam, ab99302); rabbit anti-TH1 (1:200, Abcam, ab137869); rabbit anti-TCF4 (1:100, Cell Signalling; C48H11); rabbit anti-vasopressin (1:500, Abcam, ab213707). .. Slides were then washed 3 times for 10 min each in PBT and incubated for 1 h at room temperature with fluorescent secondary antibodies in PBT in the dark, secondaries as follows: goat anti-rabbit 488 (1:500, Thermo Fisher, A11034); goat anti-mouse 488 (1:500, Thermo Fisher, A11029); donkey anti-sheep 594 (1:500, Thermo Fisher, A11016).

    Blocking Assay:

    Article Title: Surface-Engineered Umbilical Cord Mesenchymal Stem Cell-Derived sEVs for Targeted Therapy of Osteoarthritis.
    Article Snippet: Small extracellular vesicles (sEVs) from human umbilical-cord-derived mesenchymal stem cells (UCMSCs) hold promise for cartilage regeneration in osteoarthritis (OA).. However, their therapeutic effectiveness is significantly limited by rapid clearance through blood and lymphatic vessels in the synovial tissue after intra-articular injection.. This study aimed to enhance the chondrocyte-targeting ability and improve the cartilage repair potential of UCMSC-derived sEVs by conjugating them with a chondrocyte-affinity peptide (CAP) using copper-free click chemistry.

    Article Title: A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development
    Article Snippet: .. Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400, Santa Cruz, sc-52659); rabbit anti-ISL1 (1:200, Abcam, ab20670); rabbit anti-LHX3 (1:250, Abcam, ab14555); rabbit anti-NKX2-1 (1:250, Abcam, ab76013); rabbit anti-NKX2-2 (1:250, Abcam, ab191077); rabbit anti-NKX6-1 (1:250, Abcam, ab221549); rabbit anti-OLIG2 (1:200, Abcam, ab109186); rabbit anti-oxytocin (1:500, Abcam, ab212193); rabbit anti-PAX6 (1:350, Abcam, ab195045); sheep anti-PITX2 (1:200, R&D, AF7388); rabbit anti-SF1 (1:200, Abcam, ab65815); rabbit anti-TBX3 (1:200, Abcam, ab99302); rabbit anti-TH1 (1:200, Abcam, ab137869); rabbit anti-TCF4 (1:100, Cell Signalling; C48H11); rabbit anti-vasopressin (1:500, Abcam, ab213707). .. Slides were then washed 3 times for 10 min each in PBT and incubated for 1 h at room temperature with fluorescent secondary antibodies in PBT in the dark, secondaries as follows: goat anti-rabbit 488 (1:500, Thermo Fisher Scientific, A11034); goat anti-mouse 488 (1:500, Thermo Fisher Scientific, A11029); donkey anti-sheep 594 (1:500, Thermo Fisher Scientific, A11016).

    Article Title: A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development
    Article Snippet: .. For PITX2 staining, donkey serum was used, Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400, Santa Cruz, sc-52659); rabbit anti-ISL1 (1:200, Abcam, ab20670); rabbit anti-LHX3 (1:250, Abcam, ab14555); rabbit anti-NKX2-1 (1:250, Abcam, ab76013); rabbit anti-NKX2-2 (1:250, Abcam, ab191077); rabbit anti-NKX6-1 (1:250, Abcam, ab221549); rabbit anti-OLIG2 (1:200, Abcam, ab109186); rabbit anti-oxytocin (1:500, Abcam, ab212193); rabbit anti-PAX6 (1:350, Abcam, ab195045); sheep anti-PITX2 (1:200, R&D, AF7388); rabbit anti-SF1 (1:200, Abcam, ab65815); rabbit anti-TBX3 (1:200, Abcam, ab99302); rabbit anti-TH1 (1:200, Abcam, ab137869); rabbit anti-TCF4 (1:100, Cell Signalling; C48H11); rabbit anti-vasopressin (1:500, Abcam, ab213707). .. Slides were then washed 3 times for 10 min each in PBT and incubated for 1 h at room temperature with fluorescent secondary antibodies in PBT in the dark, secondaries as follows: goat anti-rabbit 488 (1:500, Thermo Fisher, A11034); goat anti-mouse 488 (1:500, Thermo Fisher, A11029); donkey anti-sheep 594 (1:500, Thermo Fisher, A11016).

    other:

    Article Title: Igf1 Regulates Fibrocartilage Stem Cells, Cartilage Growth, and Homeostasis in the Temporomandibular Joint of Mice.
    Article Snippet: Temporomandibular joint (TMJ) growth requires orchestrated interactions between various cell types.. Recent studies revealed that fibrocartilage stem cells (FCSCs) in the TMJ cartilage play critical roles as cell resources for joint development and repair.. However, the detailedmolecular network that influences FCSC fate during TMJ cartilage development remains to be elucidated.

    Staining:

    Article Title: A Highly Conserved Shh Enhancer Coordinates Hypothalamic and Craniofacial Development
    Article Snippet: .. For PITX2 staining, donkey serum was used, Slides were incubated in primary antibodies diluted in blocking solution overnight at 4°C as follows: rabbit anti-CASP3 (1:500, Abcam, ab49822); mouse anti-COL2A1 (1:400, Santa Cruz, sc-52659); rabbit anti-ISL1 (1:200, Abcam, ab20670); rabbit anti-LHX3 (1:250, Abcam, ab14555); rabbit anti-NKX2-1 (1:250, Abcam, ab76013); rabbit anti-NKX2-2 (1:250, Abcam, ab191077); rabbit anti-NKX6-1 (1:250, Abcam, ab221549); rabbit anti-OLIG2 (1:200, Abcam, ab109186); rabbit anti-oxytocin (1:500, Abcam, ab212193); rabbit anti-PAX6 (1:350, Abcam, ab195045); sheep anti-PITX2 (1:200, R&D, AF7388); rabbit anti-SF1 (1:200, Abcam, ab65815); rabbit anti-TBX3 (1:200, Abcam, ab99302); rabbit anti-TH1 (1:200, Abcam, ab137869); rabbit anti-TCF4 (1:100, Cell Signalling; C48H11); rabbit anti-vasopressin (1:500, Abcam, ab213707). .. Slides were then washed 3 times for 10 min each in PBT and incubated for 1 h at room temperature with fluorescent secondary antibodies in PBT in the dark, secondaries as follows: goat anti-rabbit 488 (1:500, Thermo Fisher, A11034); goat anti-mouse 488 (1:500, Thermo Fisher, A11029); donkey anti-sheep 594 (1:500, Thermo Fisher, A11016).



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    Developmental Studies Hybridoma Bank mouse anti col2a1 monoclonal antibody
    (A) Limb amputation in a froglet resulted in hypomorphic limb regeneration at 28 dpa. (B) Longitudinal adjacent sections of a spike regenerate at 28 dpa were underwent hematoxylin, eosin and alcian blue (HE+AB) staining or in situ hybridization with a <t>col2a1</t> antisense probe. d, distal; p, proximal. Newt (C), tadpole (D) and froglet (E) blastemas at the EB, MB and LB stages. Note that blastemas and stumps are outlined dotted black lines in (D). A dotted white line or a pair of red lines, the amputation level. Bars = 2 mm for (A), 400 μm for (B) and 1 mm for (C, D, E).
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    Image Search Results


    (A) Limb amputation in a froglet resulted in hypomorphic limb regeneration at 28 dpa. (B) Longitudinal adjacent sections of a spike regenerate at 28 dpa were underwent hematoxylin, eosin and alcian blue (HE+AB) staining or in situ hybridization with a col2a1 antisense probe. d, distal; p, proximal. Newt (C), tadpole (D) and froglet (E) blastemas at the EB, MB and LB stages. Note that blastemas and stumps are outlined dotted black lines in (D). A dotted white line or a pair of red lines, the amputation level. Bars = 2 mm for (A), 400 μm for (B) and 1 mm for (C, D, E).

    Journal: bioRxiv

    Article Title: Excessive chondrogenesis in the blastema initiates during hypomorphic limb regeneration in Xenopus froglet, but not during patterned limb regeneration in Xenopus tadpoles and newts

    doi: 10.1101/2025.03.29.646070

    Figure Lengend Snippet: (A) Limb amputation in a froglet resulted in hypomorphic limb regeneration at 28 dpa. (B) Longitudinal adjacent sections of a spike regenerate at 28 dpa were underwent hematoxylin, eosin and alcian blue (HE+AB) staining or in situ hybridization with a col2a1 antisense probe. d, distal; p, proximal. Newt (C), tadpole (D) and froglet (E) blastemas at the EB, MB and LB stages. Note that blastemas and stumps are outlined dotted black lines in (D). A dotted white line or a pair of red lines, the amputation level. Bars = 2 mm for (A), 400 μm for (B) and 1 mm for (C, D, E).

    Article Snippet: Primary antibodies were mouse anti-Msx1/2 monoclonal antibody (Developmental Studies Hybridoma Bank, 4G1-c, diluted 1:50), rabbit ant-Sox9 polyclonal antibody (Merck Millipore, AB5535, diluted 1:500) and mouse anti-Col2a1 monoclonal antibody (Developmental Studies Hybridoma Bank, II-II6B3, diluted 1:50).

    Techniques: Staining, In Situ Hybridization

    Incidence (%) of change point detection and box-and-whisker plot showing change point value using anti-Sox9 (A) or anti-Col2a1 antibody (B). In each sample group, vertical lines indicate the minimum, first quartile, median, third quartile, and maximum values, from left to right. X, the mean value; n, number of blastemas used for change point analysis.

    Journal: bioRxiv

    Article Title: Excessive chondrogenesis in the blastema initiates during hypomorphic limb regeneration in Xenopus froglet, but not during patterned limb regeneration in Xenopus tadpoles and newts

    doi: 10.1101/2025.03.29.646070

    Figure Lengend Snippet: Incidence (%) of change point detection and box-and-whisker plot showing change point value using anti-Sox9 (A) or anti-Col2a1 antibody (B). In each sample group, vertical lines indicate the minimum, first quartile, median, third quartile, and maximum values, from left to right. X, the mean value; n, number of blastemas used for change point analysis.

    Article Snippet: Primary antibodies were mouse anti-Msx1/2 monoclonal antibody (Developmental Studies Hybridoma Bank, 4G1-c, diluted 1:50), rabbit ant-Sox9 polyclonal antibody (Merck Millipore, AB5535, diluted 1:500) and mouse anti-Col2a1 monoclonal antibody (Developmental Studies Hybridoma Bank, II-II6B3, diluted 1:50).

    Techniques: Whisker Assay